DNA Extraction and Purification
Biotium Cytokeratin, HMW(KRTH/1076), CF740 conjugate, 0.1mg/mL
This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1); 64 kDa (CK3); 59 kDa (CK4); 58 kDa (CK5); 56 kDa (CK6); 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Biochain Institute Inc Genomic DNA - Multiple Sclerosis Disease: Brain: Pons, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc FFPE Tissue DNA Extraction Kit — Magnetic Beads, 1 kit/pk
BioChain’s FFPE Tissue DNA Extraction Kit - Magnetic Beads allows for facile and efficient deoxyribonucleic acid extraction from FFPE tissues, with potential high throughput capabilities and full compatibility for down-stream applications such as qPCR. Utilizing heat and proteinase K treatment, BioChain’s FFPE Tissue DNA Extraction Kit- Magnetic beads is optimized in the removal of paraffin, artial reversal of formalin cross linking, and release of DNA from fixed tissues. After clean up with DNA inding beads, the concentration of the high urity DNA can be determined by spectrophotometer or anodrop equipment.
Magbio Genomics Inc HighPrep Plasmid DNA Kit (384 preps)
Magbio's HighPrep™ Plasmid DNA kit is a magnetic beads based plasmid purification system for high throughput rapid isolation of high and low copy number plasmid DNA, BACs, PACs Cosmid and Fosmids. The system can be easily automated on many liquid handling workstation providing robustness and high quality plasmid DNA for subsequent downstream applications such as digestion and fluorescent DNA sequencing
Enzo Life Sciences pJY2 plasmid (5µg)
The AGA arginine codon is rarely used in E. coli but is common in eukaryotic genes. The low level of tRNAUCUArg can lead to low expression and misincorporation of lysine for arginine, during expression of genes containing AGA codons in E. coli. E. coli harboring pJY2 can be employed to suppress lysine misincorporation and achieve high-level expression of genes cloned into pETvectors without modification of AGA codons in the target gene sequence. pJY2 encodes tRNAUCUArg (to suppress lysine misincorporation at AGA codons) and T7 lysozyme (to depress constitutive expression of the cloned gene). For example, pJY2 allows for faithful expression of pET3a-encoded mutant ubiquitins with a high density of AGA codons (up to 14% of total codons). pJY2 can also be used as a source of tRNAUCUArg with expression systems, such as the pGEX series of vectors, that do not rely on T7 polymerase. Formulation: Liquid. In 10mM TRIS, pH 8.0, containing 1mM EDTA. Long Term Storage: -20°C.
Biochain Institute Inc Express Cloning Checker Kit II, 100 rxn/PK
BioChain's Express Cloning Checker Kits provide highly efficient methods of identifying recombinant colonies after transformation eliminating time-consuming plasmid preparation. In the large-scale screen method, the partial colonies of E. coli bacteria can be directly transfered from the transformation plates into the solutions of the kits and immediately run on an agarose gel electrophoresis. The results will be known within 1-2 hours instead of the 2 days required in a conventional method of labor-intensive plasmid DNA miniprep or colony hybridization.
New England Biolabs, Inc. Monarch® gDNA Tissue Lysis Buffer - 34 ml
The Monarch Tissue Lysis Buffer is a component of the Monarch Genomic DNA Purification Kit. It provides lysis conditions that enable rapid digestion of tissue pieces while simultaneously ensuring the genomic DNA fragment length is optimal for binding and elution. Coupled with optimized Proteinase K digestion, the tissue lysis system facilitates above-average yields for common animal tissues , including tissues that prove difficult for many other commercial kits (e.g., brain and muscle).
- A component of the Monarch Genomic DNA Purification Kit
- Provides lysis conditions for rapid digestion of tissue while ensuring optimal DNA fragment length
- Coupled with Proteinase K digestion, enables above-average yields for common animal tissues
- Monarch kit components are also available separately for added convenience
Research Products International Corp EZ DNA Methylation-Gold Kit, 50 Rxns
The EZ DNA Methylation-Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses heat denaturation instead of chemical denaturation of the input DNA. This allows for the denaturation and bisulfite conversion steps to be consolidated into one step, leading to a much-reduced incubation time. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
Complete bisulfite conversion of DNA in less than 3 hours.
Desulphonation and recovery of bisulfite-treated DNA with a spin column.
Recovered DNA is ideal for downstream analyses such as PCR, endonuclease digestion, sequencing, microarrays, etc.
Qiagen, Inc. LARGE CONSTRUCT KIT
QIAGEN Large-Construct Kit,For purification of BAC,PAC,P1,or cosmid DNA,free of genomic DNA (10), 10 QIAGEN-tip 500,Reagents,Buffers,ATP-Dependent Exonuclease,For purification of BAC,PAC,P1,or cosmid DNA,free of genomic DNA
ATCC Quantitative Genomic DNA from; Enterobacter cloacae; subsp. cloacae; Strain CDC 442-68
ATCC Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.
Biochain Institute Inc Genomic DNA - Lupus: Uterus, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Omega Biotek Inc Mag-Bind SeqDTR, Magnetic Bead-based purification system, 500 mL, Manual or automated, 96-well, 384-well, Over 800 bps
The Omega Bio-tek Mag-Bind SeqDTR is a magnetic bead-based purification system designed for the efficient and reliable removal of unincorporated dye terminators from sequencing reactions. This product employs proprietary chemistry and utilizes the unique properties of paramagnetic beads, enhancing sample purity and quality in sequencing applications. It is suitable for both manual and automated workflows, allowing users to achieve optimal performance with ease.
- Application: Removal of unincorporated dye terminators from sequencing reactions
- Technology: Magnetic bead based
- Kit volume: 500 mL
- Automation compatibility: Manual or automated
- Average read lengths: Over 800 bps (Min Phred 20)
- Reduce reagent usage: 1/16 or 1/32 compared to sephadex cleanups
- Contaminants removed: Nucleotides, primers, salts, dye terminators
- Storage conditions: 2-8 °C
- Processing time: Less than 25 minutes for 4 plates
Biochain Institute Inc Genomic DNA - Human Diabetic Diseased Tissue: Stomach, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.